FineTest
SKU:ER0935
Rat F1+2(Prothrombin Fragment 1+2) ELISA Kit
Rat F1+2(Prothrombin Fragment 1+2) ELISA Kit
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Product Name
Rat F1+2(Prothrombin Fragment 1+2) ELISA Kit
Alias
F1+2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
F1+2 ELISA Kit allows for the in vitro quantitative determination of F1+2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
46.875-3000pg/ml
Sensitivity
< 28.125pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of F1+2 and the recovery rates were calculated by comparing the measured value to the expected amount of F1+2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
96 |
|
EDTA plasma(n=5) |
87-101 |
93 |
|
heparin plasma(n=5) |
88-103 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F1+2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-105% |
86-101% |
90-104% |
|
EDTA plasma(n=5) |
93-101% |
82-98% |
83-99% |
|
heparin plasma(n=5) |
91-99% |
83-97% |
87-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
