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Rat ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit
Rat ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit
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Product Name
Rat ESM1(Endothelial Cell Specific Molecule 1) ELISA Kit
Alias
ESM1 ELISA Kit, ESM-1 ELISA Kit, IGFBP-rp6 ELISA Kit, endocan ELISA Kit, endothelial cell-specific molecule 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ESM1 ELISA Kit allows for the in vitro quantitative determination of ESM1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ESM1 and the recovery rates were calculated by comparing the measured value to the expected amount of ESM1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-102 |
97 |
|
EDTA plasma(n=5) |
91-104 |
97 |
|
heparin plasma(n=5) |
89-100 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ESM1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-99% |
91-105% |
88-100% |
|
EDTA plasma(n=5) |
85-100% |
82-101% |
83-101% |
|
heparin plasma(n=5) |
91-99% |
80-98% |
84-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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