FineTest
SKU:ER0851
Rat COL1α2(Collagen Type I Alpha 2) ELISA Kit
Rat COL1α2(Collagen Type I Alpha 2) ELISA Kit
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Product Name
Rat COL1α2(Collagen Type I Alpha 2) ELISA Kit
Alias
COL1α2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
COL1α2 ELISA Kit allows for the in vitro quantitative determination of COL1α2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of COL1α2 and the recovery rates were calculated by comparing the measured value to the expected amount of COL1α2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-105 |
94 |
|
EDTA plasma(n=5) |
87-102 |
94 |
|
heparin plasma(n=5) |
89-101 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL1α2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-103% |
91-104% |
85-104% |
|
EDTA plasma(n=5) |
91-100% |
85-100% |
85-91% |
|
heparin plasma(n=5) |
85-97% |
87-99% |
88-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
