FineTest
SKU:ER0850
Rat COL-1α1(Collagen Iα1) ELISA Kit
Rat COL-1α1(Collagen Iα1) ELISA Kit
Couldn't load pickup availability
Product Name
Rat COL-1α1(Collagen Iα1) ELISA Kit
Alias
COL-1α1 ELISA Kit, COL1α1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
COL-1α1 ELISA Kit allows for the in vitro quantitative determination of COL-1α1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of COL-1α1 and the recovery rates were calculated by comparing the measured value to the expected amount of COL-1α1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-101 |
95 |
|
EDTA plasma(n=5) |
89-104 |
95 |
|
heparin plasma(n=5) |
88-102 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL-1α1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
87-105% |
93-103% |
|
EDTA plasma(n=5) |
83-99% |
84-98% |
85-95% |
|
heparin plasma(n=5) |
86-98% |
82-93% |
83-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
