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SKU:ER0817
Rat CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit
Rat CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit
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Product Name
Rat CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit
Alias
CD40L ELISA Kit, TNFSF5 ELISA Kit, TNFSF5 ELISA Kit, CD40LG ELISA Kit, CD154 ELISA Kit, HIGM1 ELISA Kit, IGM ELISA Kit, IMD3 ELISA Kit, T-BAM ELISA Kit, TRAP ELISA Kit, Gp39 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
CD40L ELISA Kit allows for the in vitro quantitative determination of CD40L concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of CD40L and the recovery rates were calculated by comparing the measured value to the expected amount of CD40L in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-93 |
92 |
|
EDTA plasma(n=5) |
85-98 |
91 |
|
heparin plasma(n=5) |
87-105 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD40L and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-100% |
86-100% |
85-104% |
|
EDTA plasma(n=5) |
86-101% |
83-91% |
82-97% |
|
heparin plasma(n=5) |
85-98% |
85-100% |
80-91% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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