FineTest
SKU:ER0814
Rat CD28(Cluster of Differentiation 28) ELISA Kit
Rat CD28(Cluster of Differentiation 28) ELISA Kit
Couldn't load pickup availability
Product Name
Rat CD28(Cluster of Differentiation 28) ELISA Kit
Alias
CD28 ELISA Kit, CD28 antigen ELISA Kit, CD28 antigen ELISA Kit, Tp44 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
CD28 ELISA Kit allows for the in vitro quantitative determination of CD28 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of CD28 and the recovery rates were calculated by comparing the measured value to the expected amount of CD28 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-105 |
96 |
|
EDTA plasma(n=5) |
89-104 |
94 |
|
heparin plasma(n=5) |
85-101 |
90 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD28 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
93-104% |
86-102% |
85-100% |
|
EDTA plasma(n=5) |
84-95% |
82-94% |
87-101% |
|
heparin plasma(n=5) |
80-97% |
90-100% |
81-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
