FineTest
SKU:ER0786
Rat C5a(Complement Component 5a) ELISA Kit
Rat C5a(Complement Component 5a) ELISA Kit
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Product Name
Rat C5a(Complement Component 5a) ELISA Kit
Alias
C5a ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
C5a ELISA Kit allows for the in vitro quantitative determination of C5a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of C5a and the recovery rates were calculated by comparing the measured value to the expected amount of C5a in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-100 |
95 |
|
EDTA plasma(n=5) |
86-105 |
99 |
|
heparin plasma(n=5) |
87-102 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C5a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
95-104% |
92-105% |
93-105% |
|
EDTA plasma(n=5) |
83-101% |
82-89% |
88-99% |
|
heparin plasma(n=5) |
84-99% |
81-100% |
82-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
