FineTest
SKU:ER0781
Rat C3(Complement Component 3) ELISA Kit
Rat C3(Complement Component 3) ELISA Kit
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Product Name
Rat C3(Complement Component 3) ELISA Kit
Alias
C3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
C3 ELISA Kit allows for the in vitro quantitative determination of C3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.125-200ng/ml
Sensitivity
< 1.875ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of C3 and the recovery rates were calculated by comparing the measured value to the expected amount of C3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
93-98 |
95 |
|
EDTA plasma(n=5) |
85-98 |
91 |
|
heparin plasma(n=5) |
85-102 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-101% |
86-99% |
85-105% |
|
EDTA plasma(n=5) |
83-91% |
84-101% |
82-100% |
|
heparin plasma(n=5) |
81-94% |
84-100% |
80-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
