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SKU:ER0764
Rat bFGF/FGF2(Basic Fibroblast Growth Factor) ELISA Kit
Rat bFGF/FGF2(Basic Fibroblast Growth Factor) ELISA Kit
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Product Name
Rat bFGF/FGF2(Basic Fibroblast Growth Factor) ELISA Kit
Alias
bFGF ELISA Kit, FGF2 ELISA Kit, FGF-2 ELISA Kit, FGF2AS ELISA Kit, GFG1 ELISA Kit, HBGH-2 ELISA Kit, NUDT6 ELISA Kit, Prostatropin ELISA Kit, Basic fibroblast growth factor ELISA Kit, basic fibroblast growth factor bFGF ELISA Kit, FGFB ELISA Kit, fibroblast growth factor 2 ELISA Kit, basic ELISA Kit, HBGF-2 ELISA Kit, heparin-binding growth factor 2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
bFGF/FGF2 ELISA Kit allows for the in vitro quantitative determination of bFGF/FGF2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of bFGF/FGF2 and the recovery rates were calculated by comparing the measured value to the expected amount of bFGF/FGF2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-102 |
94 |
|
EDTA plasma(n=5) |
88-97 |
93 |
|
heparin plasma(n=5) |
86-100 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of bFGF/FGF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-103% |
85-105% |
89-105% |
|
EDTA plasma(n=5) |
86-95% |
83-96% |
83-101% |
|
heparin plasma(n=5) |
81-100% |
90-99% |
81-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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