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SKU:ER0739
Rat Anti-CIIAb(Anti-Type II Collagen Antibody) ELISA Kit
Rat Anti-CIIAb(Anti-Type II Collagen Antibody) ELISA Kit
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Product Name
Rat Anti-CIIAb(Anti-Type II Collagen Antibody) ELISA Kit
Alias
Anti-CIIAb ELISA Kit
Detection Method
Sandwich ELISA, Double Antigen
Application
Anti-CIIAb ELISA Kit allows for the in vitro quantitative determination of Anti-CIIAb concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ug/ml
Sensitivity
< 0.094ug/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Anti-CIIAb and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-CIIAb in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-100 |
93 |
|
EDTA plasma(n=5) |
86-101 |
94 |
|
heparin plasma(n=5) |
86-99 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-CIIAb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-99% |
86-96% |
85-104% |
|
EDTA plasma(n=5) |
82-96% |
82-100% |
82-100% |
|
heparin plasma(n=5) |
82-98% |
81-97% |
84-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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