FineTest
SKU:ER0729
Rat ALT(Alanine Transaminase) ELISA Kit
Rat ALT(Alanine Transaminase) ELISA Kit
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Product Name
Rat ALT(Alanine Transaminase) ELISA Kit
Alias
ALT ELISA Kit, Alanine Transaminase ELISA Kit, ALAT ELISA Kit, SGPT ELISA Kit, alanine aminotransferase ELISA Kit, serum glutamic pyruvic transaminase ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ALT ELISA Kit allows for the in vitro quantitative determination of ALT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ALT and the recovery rates were calculated by comparing the measured value to the expected amount of ALT in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
93 |
|
EDTA plasma(n=5) |
85-101 |
93 |
|
heparin plasma(n=5) |
91-104 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-95% |
85-104% |
87-103% |
|
EDTA plasma(n=5) |
88-101% |
82-98% |
87-101% |
|
heparin plasma(n=5) |
81-99% |
83-96% |
82-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
