FineTest
SKU:ER0724
Rat AGRN(Agrin) ELISA Kit
Rat AGRN(Agrin) ELISA Kit
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Product Name
Rat AGRN(Agrin) ELISA Kit
Alias
AGRN ELISA Kit, Agrin ELISA Kit, agrin proteoglycan ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
AGRN ELISA Kit allows for the in vitro quantitative determination of AGRN concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of AGRN and the recovery rates were calculated by comparing the measured value to the expected amount of AGRN in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
97 |
|
EDTA plasma(n=5) |
85-100 |
92 |
|
heparin plasma(n=5) |
90-105 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AGRN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-104% |
93-101% |
90-104% |
|
EDTA plasma(n=5) |
83-94% |
84-101% |
85-97% |
|
heparin plasma(n=5) |
80-99% |
81-92% |
81-88% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
