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Rat Akt2(RAC-beta serine/threonine-protein kinase) ELISA Kit
Rat Akt2(RAC-beta serine/threonine-protein kinase) ELISA Kit
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Product Name
Rat Akt2(RAC-beta serine/threonine-protein kinase) ELISA Kit
Alias
AKT2 ELISA Kit, PKB beta ELISA Kit, PKBB ELISA Kit, PKBBETA ELISA Kit, PRKBB ELISA Kit, Protein kinase Akt 2 ELISA Kit, Protein kinase B beta ELISA Kit, RAC BETA ELISA Kit, RAC PK beta ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Akt2 ELISA Kit allows for the in vitro quantitative determination of Akt2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Akt2 and the recovery rates were calculated by comparing the measured value to the expected amount of Akt2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-98 |
91 |
|
EDTA plasma(n=5) |
88-102 |
95 |
|
heparin plasma(n=5) |
86-103 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Akt2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-98% |
85-104% |
85-103% |
|
EDTA plasma(n=5) |
90-99% |
82-100% |
84-98% |
|
heparin plasma(n=5) |
84-95% |
87-100% |
81-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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