FineTest
SKU:ER0697
Rat Lig1(DNA ligase 1) ELISA Kit
Rat Lig1(DNA ligase 1) ELISA Kit
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Product Name
Rat Lig1(DNA ligase 1) ELISA Kit
Alias
Lig1 ELISA Kit, Polydeoxyribonucleotide synthase [ATP] 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Lig1 ELISA Kit allows for the in vitro quantitative determination of Lig1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Lig1 and the recovery rates were calculated by comparing the measured value to the expected amount of Lig1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-103 |
94 |
|
EDTA plasma(n=5) |
88-105 |
100 |
|
heparin plasma(n=5) |
88-103 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lig1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-101% |
90-105% |
92-104% |
|
EDTA plasma(n=5) |
85-99% |
88-97% |
98-101% |
|
heparin plasma(n=5) |
88-93% |
80-95% |
83-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
