FineTest
SKU:ER0683
Rat Ste(Estrogen sulfotransferase, isoform 3) ELISA Kit
Rat Ste(Estrogen sulfotransferase, isoform 3) ELISA Kit
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Product Name
Rat Ste(Estrogen sulfotransferase, isoform 3) ELISA Kit
Alias
EST-3 ELISA Kit, Estrogen sulfotransferase ELISA Kit, isoform 3 ELISA Kit, Estrone sulfotransferase ELISA Kit, Sulfotransferase ELISA Kit, estrogen-preferring ELISA Kit, Ste ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Ste ELISA Kit allows for the in vitro quantitative determination of Ste concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Ste and the recovery rates were calculated by comparing the measured value to the expected amount of Ste in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-105 |
95 |
|
EDTA plasma(n=5) |
85-104 |
94 |
|
heparin plasma(n=5) |
90-104 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ste and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-103% |
85-105% |
86-101% |
|
EDTA plasma(n=5) |
87-99% |
84-101% |
87-97% |
|
heparin plasma(n=5) |
85-98% |
81-99% |
80-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
