FineTest
SKU:ER0667
Rat Rap1a(Ras-related protein Rap-1A) ELISA Kit
Rat Rap1a(Ras-related protein Rap-1A) ELISA Kit
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Product Name
Rat Rap1a(Ras-related protein Rap-1A) ELISA Kit
Alias
Rap1a ELISA Kit, KREV ELISA Kit, KREV-1 ELISA Kit, RAL1B ELISA Kit, RAP1A ELISA Kit, RAP1B ELISA Kit, SMGP21 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Rap1a ELISA Kit allows for the in vitro quantitative determination of Rap1a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Rap1a and the recovery rates were calculated by comparing the measured value to the expected amount of Rap1a in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-102 |
96 |
|
EDTA plasma(n=5) |
86-99 |
92 |
|
heparin plasma(n=5) |
87-103 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rap1a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-104% |
90-103% |
85-105% |
|
EDTA plasma(n=5) |
86-101% |
90-96% |
91-96% |
|
heparin plasma(n=5) |
82-97% |
90-97% |
80-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
