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Rat Huwe1(E3 ubiquitin-protein ligase HUWE1) ELISA Kit
Rat Huwe1(E3 ubiquitin-protein ligase HUWE1) ELISA Kit
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Product Name
Rat Huwe1(E3 ubiquitin-protein ligase HUWE1) ELISA Kit
Alias
Huwe1 ELISA Kit, HECT ELISA Kit, UBA and WWE domain-containing protein 1 ELISA Kit, Upstream regulatory element-binding protein 1 ELISA Kit, URE-B1 ELISA Kit, URE-binding protein 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Huwe1 ELISA Kit allows for the in vitro quantitative determination of Huwe1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.781-50ng/ml
Sensitivity
< 0.469ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Huwe1 and the recovery rates were calculated by comparing the measured value to the expected amount of Huwe1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
92-101 |
97 |
|
EDTA plasma(n=5) |
90-100 |
95 |
|
heparin plasma(n=5) |
96-101 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Huwe1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-105% |
89-101% |
87-103% |
|
EDTA plasma(n=5) |
83-96% |
89-100% |
86-101% |
|
heparin plasma(n=5) |
80-100% |
81-98% |
86-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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