FineTest
SKU:ER0656
Rat Capn5(Calpain-5) ELISA Kit
Rat Capn5(Calpain-5) ELISA Kit
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Product Name
Rat Capn5(Calpain-5) ELISA Kit
Alias
Capn5 ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
Capn5 ELISA Kit allows for the in vitro quantitative determination of Capn5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.781-50ng/ml
Sensitivity
< 0.469ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Capn5 and the recovery rates were calculated by comparing the measured value to the expected amount of Capn5 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-99 |
92 |
|
EDTA plasma(n=5) |
85-102 |
96 |
|
heparin plasma(n=5) |
85-100 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Capn5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-101% |
86-98% |
90-103% |
|
EDTA plasma(n=5) |
86-94% |
85-97% |
89-97% |
|
heparin plasma(n=5) |
83-98% |
84-99% |
84-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
