FineTest
SKU:ER0658
Rat Kl(Klotho) ELISA Kit
Rat Kl(Klotho) ELISA Kit
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Product Name
Rat Kl(Klotho) ELISA Kit
Alias
Kl ELISA Kit, KL ELISA Kit, EC 3.2.1 ELISA Kit, EC 3.2.1.31 ELISA Kit, klotho ELISA Kit, KLOTHO ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Kl ELISA Kit allows for the in vitro quantitative determination of Kl concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Kl and the recovery rates were calculated by comparing the measured value to the expected amount of Kl in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
94-104 |
99 |
|
EDTA plasma(n=5) |
88-104 |
98 |
|
heparin plasma(n=5) |
88-105 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Kl and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-97% |
86-103% |
85-104% |
|
EDTA plasma(n=5) |
85-101% |
90-99% |
83-90% |
|
heparin plasma(n=5) |
81-98% |
80-98% |
80-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
