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SKU:ER0601

Rat Shh(Sonic hedgehog protein) ELISA Kit

Rat Shh(Sonic hedgehog protein) ELISA Kit

Regular price $450.00 USD
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size
Sensitivity
Range

Product Name

Rat Shh(Sonic hedgehog protein) ELISA Kit

Alias

Shh ELISA Kit, HHG1 ELISA Kit, HHG-1 ELISA Kit, HLP3 ELISA Kit, HPE3 ELISA Kit, SMMCIsonic hedgehog homolog ELISA Kit, Drosophila ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

Shh ELISA Kit allows for the in vitro quantitative determination of Shh concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Rat

UniProt ID

Q63673

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of Shh and the recovery rates were calculated by comparing the measured value to the expected amount of Shh in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

87-102

93

EDTA plasma(n=5)

87-104

92

heparin plasma(n=5)

95-104

100

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Shh and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

89-105%

86-105%

92-97%

EDTA plasma(n=5)

85-92%

84-88%

84-100%

heparin plasma(n=5)

90-98%

80-100%

81-99%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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