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SKU:ER0563

Rat Fndc5(Fibronectin type III domain-containing protein 5) ELISA Kit

Rat Fndc5(Fibronectin type III domain-containing protein 5) ELISA Kit

Regular price $450.00 USD
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size
Sensitivity
Range

Product Name

Rat Fndc5(Fibronectin type III domain-containing protein 5) ELISA Kit

Alias

Fndc5 ELISA Kit, Fibronectin type III domain-containing protein 5 ELISA Kit, Fibronectin type III repeat-containing protein 2 ELISA Kit, Irisin ELISA Kit, FRCP2 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

Fndc5 ELISA Kit allows for the in vitro quantitative determination of Fndc5 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

62.5-4000pg/ml

Sensitivity

< 37.5pg/ml

Species

Rat

UniProt ID

Q8K3V5

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of Fndc5 and the recovery rates were calculated by comparing the measured value to the expected amount of Fndc5 in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

88-104

98

EDTA plasma(n=5)

86-105

95

heparin plasma(n=5)

88-98

95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fndc5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

88-102%

85-100%

86-98%

EDTA plasma(n=5)

84-97%

87-98%

84-98%

heparin plasma(n=5)

85-100%

81-97%

83-99%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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