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SKU:ER0563
Rat Fndc5(Fibronectin type III domain-containing protein 5) ELISA Kit
Rat Fndc5(Fibronectin type III domain-containing protein 5) ELISA Kit
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Product Name
Rat Fndc5(Fibronectin type III domain-containing protein 5) ELISA Kit
Alias
Fndc5 ELISA Kit, Fibronectin type III domain-containing protein 5 ELISA Kit, Fibronectin type III repeat-containing protein 2 ELISA Kit, Irisin ELISA Kit, FRCP2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Fndc5 ELISA Kit allows for the in vitro quantitative determination of Fndc5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
62.5-4000pg/ml
Sensitivity
< 37.5pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Fndc5 and the recovery rates were calculated by comparing the measured value to the expected amount of Fndc5 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-104 |
98 |
|
EDTA plasma(n=5) |
86-105 |
95 |
|
heparin plasma(n=5) |
88-98 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fndc5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-102% |
85-100% |
86-98% |
|
EDTA plasma(n=5) |
84-97% |
87-98% |
84-98% |
|
heparin plasma(n=5) |
85-100% |
81-97% |
83-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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