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SKU:ER0562
Rat Hspa5(78 kDa glucose-regulated protein) ELISA Kit
Rat Hspa5(78 kDa glucose-regulated protein) ELISA Kit
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Product Name
Rat Hspa5(78 kDa glucose-regulated protein) ELISA Kit
Alias
Hspa5 ELISA Kit, GRP-78 ELISA Kit, Grp78 ELISA Kit, Steroidogenesis-activator polypeptide ELISA Kit, Heat shock 70 kDa protein 5 ELISA Kit, Immunoglobulin heavy chain-binding protein ELISA Kit, BiP ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Hspa5 ELISA Kit allows for the in vitro quantitative determination of Hspa5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Hspa5 and the recovery rates were calculated by comparing the measured value to the expected amount of Hspa5 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-99 |
93 |
|
EDTA plasma(n=5) |
90-105 |
98 |
|
heparin plasma(n=5) |
86-105 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hspa5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-103% |
91-101% |
86-103% |
|
EDTA plasma(n=5) |
84-99% |
85-101% |
83-97% |
|
heparin plasma(n=5) |
91-98% |
80-99% |
81-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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