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SKU:ER0562

Rat Hspa5(78 kDa glucose-regulated protein) ELISA Kit

Rat Hspa5(78 kDa glucose-regulated protein) ELISA Kit

Regular price $450.00 USD
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size
Sensitivity
Range

Product Name

Rat Hspa5(78 kDa glucose-regulated protein) ELISA Kit

Alias

Hspa5 ELISA Kit, GRP-78 ELISA Kit, Grp78 ELISA Kit, Steroidogenesis-activator polypeptide ELISA Kit, Heat shock 70 kDa protein 5 ELISA Kit, Immunoglobulin heavy chain-binding protein ELISA Kit, BiP ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

Hspa5 ELISA Kit allows for the in vitro quantitative determination of Hspa5 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Species

Rat

UniProt ID

P06761

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of Hspa5 and the recovery rates were calculated by comparing the measured value to the expected amount of Hspa5 in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

86-99

93

EDTA plasma(n=5)

90-105

98

heparin plasma(n=5)

86-105

99

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hspa5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

90-103%

91-101%

86-103%

EDTA plasma(n=5)

84-99%

85-101%

83-97%

heparin plasma(n=5)

91-98%

80-99%

81-93%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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