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SKU:ER0533

Rat Pola2(DNA polymerase alpha subunit B) ELISA Kit

Rat Pola2(DNA polymerase alpha subunit B) ELISA Kit

Regular price $450.00 USD
Regular price Sale price $450.00 USD
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size
Sensitivity
Range

Product Name

Rat Pola2(DNA polymerase alpha subunit B) ELISA Kit

Alias

Pola2 ELISA Kit, DNA polymerase alpha subunit B ELISA Kit, DNA polymerase alpha 70 kDa subunit ELISA Kit, DNA polymerase subunit II ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

Pola2 ELISA Kit allows for the in vitro quantitative determination of Pola2 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Species

Rat

UniProt ID

O89043

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of Pola2 and the recovery rates were calculated by comparing the measured value to the expected amount of Pola2 in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

94-97

95

EDTA plasma(n=5)

95-104

98

heparin plasma(n=5)

86-105

96

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pola2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

86-99%

87-105%

85-105%

EDTA plasma(n=5)

84-93%

85-100%

86-95%

heparin plasma(n=5)

84-100%

84-99%

84-100%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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