FineTest
SKU:ER0537
Rat Col27a1(Collagen alpha-1(XXVII) chain) ELISA Kit
Rat Col27a1(Collagen alpha-1(XXVII) chain) ELISA Kit
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Product Name
Rat Col27a1(Collagen alpha-1(XXVII) chain) ELISA Kit
Alias
Col27a1 ELISA Kit, Collagen alpha-1 ELISA Kit, XXVII chain ELISA Kit, FLJ11895 ELISA Kit, MGC11337 ELISA Kit, KIAA1870 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Col27a1 chain) ELISA Kit allows for the in vitro quantitative determination of Col27a1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.56-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Col27a1 and the recovery rates were calculated by comparing the measured value to the expected amount of Col27a1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-105 |
96 |
|
EDTA plasma(n=5) |
87-103 |
94 |
|
heparin plasma(n=5) |
89-103 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Col27a1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-98% |
86-103% |
88-105% |
|
EDTA plasma(n=5) |
82-99% |
88-99% |
86-97% |
|
heparin plasma(n=5) |
93-98% |
83-99% |
87-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
