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SKU:ER0514
Rat Adcyap1(Pituitary adenylate cyclase-activating polypeptide) ELISA Kit
Rat Adcyap1(Pituitary adenylate cyclase-activating polypeptide) ELISA Kit
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Product Name
Rat Adcyap1(Pituitary adenylate cyclase-activating polypeptide) ELISA Kit
Alias
Adcyap1 ELISA Kit, PACAP ELISA Kit, Pituitary adenylate cyclase-activating polypeptide ELISA Kit, ADCYAP1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Adcyap1 ELISA Kit allows for the in vitro quantitative determination of Adcyap1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
6.25-400pg/ml
Sensitivity
< 3.75pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Adcyap1 and the recovery rates were calculated by comparing the measured value to the expected amount of Adcyap1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-105 |
95 |
|
EDTA plasma(n=5) |
91-96 |
93 |
|
heparin plasma(n=5) |
95-104 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Adcyap1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-101% |
90-103% |
85-104% |
|
EDTA plasma(n=5) |
84-94% |
92-98% |
88-101% |
|
heparin plasma(n=5) |
82-93% |
80-100% |
80-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
