FineTest
SKU:ER0505
Rat Ppme1(Protein phosphatase methylesterase 1) ELISA Kit
Rat Ppme1(Protein phosphatase methylesterase 1) ELISA Kit
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Product Name
Rat Ppme1(Protein phosphatase methylesterase 1) ELISA Kit
Alias
Ppme1 ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
Ppme1 ELISA Kit allows for the in vitro quantitative determination of Ppme1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.625-40ng/ml
Sensitivity
< 0.375ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Ppme1 and the recovery rates were calculated by comparing the measured value to the expected amount of Ppme1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-101 |
91 |
|
EDTA plasma(n=5) |
85-104 |
93 |
|
heparin plasma(n=5) |
91-103 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ppme1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-103% |
85-103% |
89-104% |
|
EDTA plasma(n=5) |
82-101% |
82-94% |
89-100% |
|
heparin plasma(n=5) |
85-99% |
83-99% |
80-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
