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SKU:ER0483
Rat Gad1(Glutamate decarboxylase 1) ELISA Kit
Rat Gad1(Glutamate decarboxylase 1) ELISA Kit
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Product Name
Rat Gad1(Glutamate decarboxylase 1) ELISA Kit
Alias
Gad1 ELISA Kit, GAD67 ELISA Kit, 67kD ELISA Kit, EC 4.1.1 ELISA Kit, FLJ45882 ELISA Kit, GAD25 ELISA Kit, glutamate decarboxylase 1 ELISA Kit, brain ELISA Kit, 67kDa ELISA Kit, Glutamate decarboxylase 67 kDa isoform ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Gad1 ELISA Kit allows for the in vitro quantitative determination of Gad1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.391-25ng/ml
Sensitivity
< 0.234ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Gad1 and the recovery rates were calculated by comparing the measured value to the expected amount of Gad1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-99 |
94 |
|
EDTA plasma(n=5) |
88-101 |
96 |
|
heparin plasma(n=5) |
89-103 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gad1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-101% |
91-99% |
88-105% |
|
EDTA plasma(n=5) |
83-101% |
82-95% |
83-99% |
|
heparin plasma(n=5) |
80-93% |
84-94% |
85-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
