FineTest
SKU:ER0476
Rat Dio3(Type III iodothyronine deiodinase) ELISA Kit
Rat Dio3(Type III iodothyronine deiodinase) ELISA Kit
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Product Name
Rat Dio3(Type III iodothyronine deiodinase) ELISA Kit
Alias
Dio3 ELISA Kit, Type-III 5'-deiodinase ELISA Kit, Type 3 DI ELISA Kit, 5DIII ELISA Kit, DIOIII ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Dio3 ELISA Kit allows for the in vitro quantitative determination of Dio3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Dio3 and the recovery rates were calculated by comparing the measured value to the expected amount of Dio3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-98 |
91 |
|
EDTA plasma(n=5) |
85-101 |
95 |
|
heparin plasma(n=5) |
92-101 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Dio3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-105% |
87-104% |
88-105% |
|
EDTA plasma(n=5) |
84-98% |
82-98% |
86-95% |
|
heparin plasma(n=5) |
82-100% |
80-97% |
80-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
