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SKU:ER0436
Rat P4ha1(Prolyl 4-hydroxylase subunit alpha-1) ELISA Kit
Rat P4ha1(Prolyl 4-hydroxylase subunit alpha-1) ELISA Kit
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Product Name
Rat P4ha1(Prolyl 4-hydroxylase subunit alpha-1) ELISA Kit
Alias
P4ha1 ELISA Kit, PHalphaI ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
P4ha1 ELISA Kit allows for the in vitro quantitative determination of P4ha1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of P4ha1 and the recovery rates were calculated by comparing the measured value to the expected amount of P4ha1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-96 |
91 |
|
EDTA plasma(n=5) |
90-102 |
97 |
|
heparin plasma(n=5) |
85-103 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of P4ha1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-103% |
87-102% |
85-104% |
|
EDTA plasma(n=5) |
83-91% |
85-100% |
84-99% |
|
heparin plasma(n=5) |
85-99% |
82-98% |
82-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
