FineTest
SKU:ER0422
Rat Acadm(Medium-chain specific acyl-CoA dehydrogenase, mitochondrial) ELISA Kit
Rat Acadm(Medium-chain specific acyl-CoA dehydrogenase, mitochondrial) ELISA Kit
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Product Name
Rat Acadm(Medium-chain specific acyl-CoA dehydrogenase, mitochondrial) ELISA Kit
Alias
Acadm ELISA Kit, Medium-chain specific acyl-CoA dehydrogenase ELISA Kit, mitochondrial ELISA Kit, MCAD ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
Acadm ELISA Kit allows for the in vitro quantitative determination of Acadm concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.781-50ng/ml
Sensitivity
< 0.469ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Acadm and the recovery rates were calculated by comparing the measured value to the expected amount of Acadm in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-97 |
92 |
|
EDTA plasma(n=5) |
93-105 |
98 |
|
heparin plasma(n=5) |
85-98 |
90 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acadm and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-101% |
86-94% |
88-96% |
|
EDTA plasma(n=5) |
82-101% |
86-99% |
83-99% |
|
heparin plasma(n=5) |
87-96% |
81-100% |
89-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
