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SKU:ER0425

Rat Pdk1(Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial) ELISA Kit

Rat Pdk1(Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial) ELISA Kit

Regular price $450.00 USD
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size
Sensitivity
Range

Product Name

Rat Pdk1(Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial) ELISA Kit

Alias

Pdk1 ELISA Kit, PDK1 ELISA Kit, PDPK1 ELISA Kit, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1 ELISA Kit, mitochondrial ELISA Kit, mitochondrial pyruvate dehydrogenase ELISA Kit, lipoamide ELISA Kit, kinase isoenzyme 1 ELISA Kit, Pyruvate dehydrogenase kinase isoform 1 ELISA Kit, pyruvate dehydrogenase kinase ELISA Kit, isoenzyme 1 ELISA Kit, pyruvate dehydrogenase kinase ELISA Kit, isozyme 1 ELISA Kit, PKB kinase ELISA Kit, PDK p48 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

Pdk1 ELISA Kit allows for the in vitro quantitative determination of Pdk1 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Rat

UniProt ID

Q63065

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of Pdk1 and the recovery rates were calculated by comparing the measured value to the expected amount of Pdk1 in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

89-103

95

EDTA plasma(n=5)

86-99

94

heparin plasma(n=5)

91-104

99

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pdk1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

85-100%

88-99%

86-105%

EDTA plasma(n=5)

82-99%

84-94%

88-101%

heparin plasma(n=5)

84-93%

81-97%

84-95%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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