FineTest
SKU:ER0385
Rat PYGB(Glycogen phosphorylase, brain form) ELISA Kit
Rat PYGB(Glycogen phosphorylase, brain form) ELISA Kit
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Product Name
Rat PYGB(Glycogen phosphorylase, brain form) ELISA Kit
Alias
PYGB ELISA Kit, Glycogen phosphorylase brain form ELISA Kit, Glycogen Phosphorylase BB ELISA Kit, GPBB ELISA Kit, Brain glycogen phosphorylase ELISA Kit, Glycogen phosphorylase B ELISA Kit, Glycogen Phosphorylase Isoenzyme BB ELISA Kit, Phosphorylase glycogen brain ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PYGB ELISA Kit allows for the in vitro quantitative determination of PYGB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PYGB and the recovery rates were calculated by comparing the measured value to the expected amount of PYGB in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-104 |
95 |
|
EDTA plasma(n=5) |
91-100 |
96 |
|
heparin plasma(n=5) |
85-97 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PYGB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-103% |
87-102% |
85-101% |
|
EDTA plasma(n=5) |
82-95% |
86-98% |
85-100% |
|
heparin plasma(n=5) |
82-96% |
82-98% |
85-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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