FineTest
SKU:ER0382
Rat ACTH(Adrenocorticotropic Hormone) ELISA Kit
Rat ACTH(Adrenocorticotropic Hormone) ELISA Kit
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Product Name
Rat ACTH(Adrenocorticotropic Hormone) ELISA Kit
Alias
ACTH ELISA Kit, Corticotropin ELISA Kit, Adrenocorticotropin ELISA Kit, Adrenocorticotropic Hormone ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
ACTH ELISA Kit allows for the in vitro quantitative determination of ACTH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ACTH and the recovery rates were calculated by comparing the measured value to the expected amount of ACTH in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-105 |
96 |
|
EDTA plasma(n=5) |
85-102 |
91 |
|
heparin plasma(n=5) |
93-101 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-102% |
92-101% |
90-98% |
|
EDTA plasma(n=5) |
82-92% |
88-94% |
83-94% |
|
heparin plasma(n=5) |
82-95% |
81-89% |
88-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
