FineTest
SKU:ER0368
Rat Hcrt(Orexin) ELISA Kit
Rat Hcrt(Orexin) ELISA Kit
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Product Name
Rat Hcrt(Orexin) ELISA Kit
Alias
Hcrt ELISA Kit, OX ELISA Kit, PPORX ELISA Kit, PPOX ELISA Kit, Orexin ELISA Kit, Hypocretin ELISA Kit, Hcrt ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Hcrt ELISA Kit allows for the in vitro quantitative determination of Hcrt concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
7.813-500pg/ml
Sensitivity
< 4.688pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Hcrt and the recovery rates were calculated by comparing the measured value to the expected amount of Hcrt in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-103 |
92 |
|
EDTA plasma(n=5) |
90-96 |
93 |
|
heparin plasma(n=5) |
88-102 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hcrt and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
98-104% |
85-102% |
85-92% |
|
EDTA plasma(n=5) |
85-99% |
83-95% |
84-98% |
|
heparin plasma(n=5) |
83-98% |
80-98% |
83-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
