FineTest
SKU:ER0363
Rat TLR4(Toll-like receptor 4) ELISA Kit
Rat TLR4(Toll-like receptor 4) ELISA Kit
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Product Name
Rat TLR4(Toll-like receptor 4) ELISA Kit
Alias
TLR4 ELISA Kit, CD284 ELISA Kit, ARMD10 ELISA Kit, CD284 antigen ELISA Kit, hTollhomolog of Drosophila toll ELISA Kit, TOLL ELISA Kit, toll-like receptor 4 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TLR4 ELISA Kit allows for the in vitro quantitative determination of TLR4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TLR4 and the recovery rates were calculated by comparing the measured value to the expected amount of TLR4 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-101 |
95 |
|
EDTA plasma(n=5) |
90-104 |
97 |
|
heparin plasma(n=5) |
86-101 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TLR4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
95-104% |
94-103% |
93-104% |
|
EDTA plasma(n=5) |
87-100% |
88-97% |
83-101% |
|
heparin plasma(n=5) |
81-99% |
81-96% |
80-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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