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Rat Pkm2(Pyruvate kinase isozymes M1/M2) ELISA Kit
Rat Pkm2(Pyruvate kinase isozymes M1/M2) ELISA Kit
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Product Name
Rat Pkm2(Pyruvate kinase isozymes M1/M2) ELISA Kit
Alias
Pkm2 ELISA Kit, Pykm ELISA Kit, Pykm ELISA Kit, Pyruvate kinase isozymes M1 ELISA Kit, M2 ELISA Kit, Pyruvate kinase muscle isozyme ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Pkm2 ELISA Kit allows for the in vitro quantitative determination of Pkm2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.25-80IU/ml
Sensitivity
< 0.75IU/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Pkm2 and the recovery rates were calculated by comparing the measured value to the expected amount of Pkm2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-100 |
94 |
|
EDTA plasma(n=5) |
86-105 |
92 |
|
heparin plasma(n=5) |
87-105 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pkm2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-105% |
86-102% |
92-105% |
|
EDTA plasma(n=5) |
87-101% |
84-100% |
83-100% |
|
heparin plasma(n=5) |
92-100% |
81-100% |
83-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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