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Rat PIIINP(N-terminal propeptide of Collagen alpha-1(III) chain) ELISA Kit
Rat PIIINP(N-terminal propeptide of Collagen alpha-1(III) chain) ELISA Kit
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Product Name
Rat PIIINP(N-terminal propeptide of procollagen type III)ELISA Kit
Alias
PIIINP ELISA Kit, P3NP ELISA Kit, N-Propeptide Of Type III Procollagen ELISA Kit, Procollagen III Amino Terminal Propeptide ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PIIINPELISA Kit allows for the in vitro quantitative determination of PIIINP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PIIINP and the recovery rates were calculated by comparing the measured value to the expected amount of PIIINP in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-103 |
96 |
|
EDTA plasma(n=5) |
85-104 |
97 |
|
heparin plasma(n=5) |
85-101 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIIINP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-99% |
97-105% |
89-102% |
|
EDTA plasma(n=5) |
85-101% |
88-100% |
89-100% |
|
heparin plasma(n=5) |
83-93% |
85-93% |
82-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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