FineTest
SKU:ER0301
Rat Pde5a(cGMP-specific 3',5'-cyclic phosphodiesterase) ELISA Kit
Rat Pde5a(cGMP-specific 3',5'-cyclic phosphodiesterase) ELISA Kit
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Product Name
Rat Pde5a(cGMP-specific 3′,5′-cyclic phosphodiesterase) ELISA KitAlias
Pde5a ELISA Kit, cGMP-binding cGMP-specific phosphodiesterase ELISA Kit, CGB-PDE ELISA Kit, PDE5 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Pde5a ELISA Kit allows for the in vitro quantitative determination of Pde5a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Pde5a and the recovery rates were calculated by comparing the measured value to the expected amount of Pde5a in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-101 |
96 |
|
EDTA plasma(n=5) |
90-105 |
98 |
|
heparin plasma(n=5) |
93-105 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pde5a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-97% |
85-101% |
85-101% |
|
EDTA plasma(n=5) |
85-101% |
83-99% |
83-96% |
|
heparin plasma(n=5) |
81-84% |
81-99% |
84-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
