FineTest
SKU:ER0299
Rat Sirt2(NAD-dependent deacetylase sirtuin-2) ELISA Kit
Rat Sirt2(NAD-dependent deacetylase sirtuin-2) ELISA Kit
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Product Name
Rat Sirt2(NAD-dependent deacetylase sirtuin-2) ELISA Kit
Alias
Sirt2 ELISA Kit, SIR2L2 ELISA Kit, sirtuin 2 ELISA Kit, sirtuin type 2 ELISA Kit, NAD-dependent deacetylase sirtuin-2 ELISA Kit, SIR2-like protein 2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Sirt2 ELISA Kit allows for the in vitro quantitative determination of Sirt2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Sirt2 and the recovery rates were calculated by comparing the measured value to the expected amount of Sirt2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-96 |
90 |
|
EDTA plasma(n=5) |
87-98 |
93 |
|
heparin plasma(n=5) |
85-104 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sirt2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-103% |
88-105% |
88-105% |
|
EDTA plasma(n=5) |
88-101% |
83-98% |
85-100% |
|
heparin plasma(n=5) |
80-96% |
87-100% |
83-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
