FineTest
SKU:ER0232
Rat Nt5e(5'-nucleotidase) ELISA Kit
Rat Nt5e(5'-nucleotidase) ELISA Kit
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Product Name
Rat Nt5e(5′-nucleotidase) ELISA Kit
Alias
Nt5e ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Nt5e ELISA Kit allows for the in vitro quantitative determination of Nt5e concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.125-200pg/ml
Sensitivity
< 1.875pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Nt5e and the recovery rates were calculated by comparing the measured value to the expected amount of Nt5e in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-105 |
95 |
|
EDTA plasma(n=5) |
94-105 |
99 |
|
heparin plasma(n=5) |
87-105 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Nt5e and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
96-105% |
85-103% |
89-103% |
|
EDTA plasma(n=5) |
85-98% |
82-98% |
84-97% |
|
heparin plasma(n=5) |
81-100% |
89-97% |
82-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
