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Rat 5-LO(Arachidonate 5-Lipoxygenase) ELISA Kit
Rat 5-LO(Arachidonate 5-Lipoxygenase) ELISA Kit
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Product Name
Rat 5-LO(Arachidonate 5-Lipoxygenase) ELISA Kit
Alias
5-LO ELISA Kit, Alox5 ELISA Kit, 5-LO ELISA Kit, 5LPG ELISA Kit, LOG5 ELISA Kit, LOX5A ELISA Kit, 5-lipoxygenase ELISA Kit, 5-LOX ELISA Kit, arachidonic 5-lipoxygenase alpha-10 isoform ELISA Kit, arachidonic 5-lipoxygenase delta-10-13 isoform ELISA Kit, arachidonic 5-lipoxygenase delta-13 isoform ELISA Kit, arachidonic 5-lipoxygenase delta-p10 isoform ELISA Kit, arachidonic acid 5-lipoxygenase ELISA Kit, leukotriene A4 synthase ELISA Kit, LOG5 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
5-LO ELISA Kit allows for the in vitro quantitative determination of 5-LO concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of 5-LO and the recovery rates were calculated by comparing the measured value to the expected amount of 5-LO in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-102 |
94 |
|
EDTA plasma(n=5) |
92-100 |
97 |
|
heparin plasma(n=5) |
86-105 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-LO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-102% |
86-105% |
86-99% |
|
EDTA plasma(n=5) |
83-99% |
89-100% |
82-95% |
|
heparin plasma(n=5) |
84-95% |
80-100% |
80-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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