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SKU:ER0191
Rat HIF-1α(Hypoxia-inducible factor 1-alpha) ELISA Kit
Rat HIF-1α(Hypoxia-inducible factor 1-alpha) ELISA Kit
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Product Name
Rat HIF-1α(Hypoxia-inducible factor 1-alpha) ELISA Kit
Alias
HIF-1α ELISA Kit, HIF1A ELISA Kit, ARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA Kit, HIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS protein 1 ELISA Kit, member of PAS superfamily 1 ELISA Kit, PAS domain-containing protein 8 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HIF-1α ELISA Kit allows for the in vitro quantitative determination of HIF-1α concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
7.813-500pg/ml
Sensitivity
< 4.688pg/ml
Species
Rat
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of HIF-1α and the recovery rates were calculated by comparing the measured value to the expected amount of HIF-1α in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-99 |
94 |
|
EDTA plasma(n=5) |
87-103 |
96 |
|
heparin plasma(n=5) |
88-99 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF-1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-105% |
89-105% |
93-103% |
|
EDTA plasma(n=5) |
82-99% |
86-99% |
86-101% |
|
heparin plasma(n=5) |
87-99% |
80-99% |
87-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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