Skip to product information
1 of 1

FineTest

SKU:ER0191

Rat HIF-1α(Hypoxia-inducible factor 1-alpha) ELISA Kit

Rat HIF-1α(Hypoxia-inducible factor 1-alpha) ELISA Kit

Regular price $400.00 USD
Regular price Sale price $400.00 USD
Sale Sold out
Shipping calculated at checkout.
size
Sensitivity
Range

Product Name

Rat HIF-1α(Hypoxia-inducible factor 1-alpha) ELISA Kit

Alias

HIF-1α ELISA Kit, HIF1A ELISA Kit, ARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA Kit, HIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS protein 1 ELISA Kit, member of PAS superfamily 1 ELISA Kit, PAS domain-containing protein 8 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

HIF-1α ELISA Kit allows for the in vitro quantitative determination of HIF-1α concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

7.813-500pg/ml

Sensitivity

< 4.688pg/ml

Species

Rat

UniProt ID

O35800

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HIF-1α and the recovery rates were calculated by comparing the measured value to the expected amount of HIF-1α in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

86-99

94

EDTA plasma(n=5)

87-103

96

heparin plasma(n=5)

88-99

92

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF-1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

85-105%

89-105%

93-103%

EDTA plasma(n=5)

82-99%

86-99%

86-101%

heparin plasma(n=5)

87-99%

80-99%

87-100%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

View full details