FineTest
SKU:ER0190
Rat Gsk3a(Glycogen synthase kinase-3 alpha) ELISA Kit
Rat Gsk3a(Glycogen synthase kinase-3 alpha) ELISA Kit
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Product Name
Rat Gsk3a(Glycogen synthase kinase-3 alpha) ELISA Kit
Alias
Gsk3a ELISA Kit, Glycogen synthase kinase-3 alpha ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Gsk3a ELISA Kit allows for the in vitro quantitative determination of Gsk3a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.625-40ng/ml
Sensitivity
< 0.375ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Gsk3a and the recovery rates were calculated by comparing the measured value to the expected amount of Gsk3a in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-104 |
94 |
|
EDTA plasma(n=5) |
92-101 |
97 |
|
heparin plasma(n=5) |
86-102 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gsk3a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-102% |
89-99% |
87-102% |
|
EDTA plasma(n=5) |
85-94% |
85-101% |
84-99% |
|
heparin plasma(n=5) |
82-95% |
81-98% |
82-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
