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ER0148

Rat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) ELISA Kit

Rat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) ELISA Kit

$450.00 USD
$450.00 USD
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size
Sensitivity
Range

Product Name

Rat NOS3/eNOS(Nitric Oxide Synthase 3, Endothelial) ELISA Kit

Alias

NOS3 ELISA Kit, eNOS ELISA Kit, Constitutive NOS ELISA Kit, ECNOS ELISA Kit, endothelial ELISA Kit, Endothelial NOS ELISA Kit, eNOS ELISA Kit, cNOS ELISA Kit, nitric oxide synthase 3 ELISA Kit, endothelial cell ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

NOS3/eNOS ELISA Kit allows for the in vitro quantitative determination of NOS3/eNOS concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Species

Rat

UniProt ID

Q62600

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of NOS3/eNOS and the recovery rates were calculated by comparing the measured value to the expected amount of NOS3/eNOS in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

91-100

97

EDTA plasma(n=5)

86-104

95

heparin plasma(n=5)

95-105

100

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS3/eNOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

88-102%

85-93%

85-96%

EDTA plasma(n=5)

85-100%

82-97%

82-101%

heparin plasma(n=5)

84-95%

85-92%

80-99%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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