FineTest
SKU:ER0115
Rat Lep(Leptin) ELISA Kit
Rat Lep(Leptin) ELISA Kit
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Product Name
Rat Lep(Leptin) ELISA Kit
Alias
LEP ELISA Kit, Leptin ELISA Kit, LEPD ELISA Kit, OBS ELISA Kit, Obesity Homolog ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Lep ELISA Kit allows for the in vitro quantitative determination of Lep concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Lep and the recovery rates were calculated by comparing the measured value to the expected amount of Lep in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-105 |
94 |
|
EDTA plasma(n=5) |
89-101 |
94 |
|
heparin plasma(n=5) |
85-103 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lep and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-97% |
89-100% |
88-104% |
|
EDTA plasma(n=5) |
89-97% |
84-100% |
84-92% |
|
heparin plasma(n=5) |
90-97% |
84-89% |
80-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
