FineTest
SKU:ER0074
Rat Afp(Alpha-fetoprotein) ELISA Kit
Rat Afp(Alpha-fetoprotein) ELISA Kit
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Product Name
Rat Afp(Alpha-fetoprotein) ELISA Kit
Alias
Afp ELISA Kit, αFP ELISA Kit, Alpha-Fetoprotein ELISA Kit, AFP ELISA Kit, FETA ELISA Kit, HPAFP ELISA Kit, oralpha fetal protein ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Afp ELISA Kit allows for the in vitro quantitative determination of Afp concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Afp and the recovery rates were calculated by comparing the measured value to the expected amount of Afp in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-100 |
93 |
|
EDTA plasma(n=5) |
88-99 |
91 |
|
heparin plasma(n=5) |
88-97 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Afp and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-97% |
89-99% |
85-97% |
|
EDTA plasma(n=5) |
83-98% |
82-101% |
85-97% |
|
heparin plasma(n=5) |
85-99% |
81-99% |
80-91% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
