FineTest
SKU:ER0055
Rat NT-3(Neurotrophin 3) ELISA Kit
Rat NT-3(Neurotrophin 3) ELISA Kit
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Product Name
Rat NT-3(Neurotrophin 3) ELISA Kit
Alias
NT-3 ELISA Kit, Neurotrophin-3 ELISA Kit, NTF3 ELISA Kit, 3-nucleotidase ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
NT-3 ELISA Kit allows for the in vitro quantitative determination of NT-3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of NT-3 and the recovery rates were calculated by comparing the measured value to the expected amount of NT-3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-103 |
98 |
|
EDTA plasma(n=5) |
87-98 |
92 |
|
heparin plasma(n=5) |
88-105 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
93-105% |
90-100% |
94-105% |
|
EDTA plasma(n=5) |
85-99% |
85-99% |
83-96% |
|
heparin plasma(n=5) |
83-98% |
84-94% |
90-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
