FineTest
SKU:ER0056
Rat PDGF-AB(Platelet-Derived Growth Factor AB) ELISA Kit
Rat PDGF-AB(Platelet-Derived Growth Factor AB) ELISA Kit
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Product Name
Rat PDGF-AB(Platelet-Derived Growth Factor AB) ELISA Kit
Alias
PDGF-AB ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PDGF-AB ELISA Kit allows for the in vitro quantitative determination of PDGF-AB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
7.813-500pg/ml
Sensitivity
< 4.688pg/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PDGF-AB and the recovery rates were calculated by comparing the measured value to the expected amount of PDGF-AB in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-101 |
95 |
|
EDTA plasma(n=5) |
91-104 |
97 |
|
heparin plasma(n=5) |
88-95 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGF-AB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-100% |
85-99% |
89-104% |
|
EDTA plasma(n=5) |
82-101% |
83-93% |
83-96% |
|
heparin plasma(n=5) |
84-94% |
81-100% |
84-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
