FineTest
SKU:ER0042
Rat IL-6(Interleukin-6) ELISA Kit
Rat IL-6(Interleukin-6) ELISA Kit
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Product Name
Rat IL-6(Interleukin-6) ELISA Kit
Alias
IL-6 ELISA Kit, Interleukin 6 ELISA Kit, IL6 ELISA Kit, BSF2 ELISA Kit, HGF ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, CDF ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-6 ELISA Kit allows for the in vitro quantitative determination of IL-6 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
62.5-4000pg/ml
Sensitivity
< 37.5pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-6 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-94 |
90 |
|
EDTA plasma(n=5) |
85-96 |
90 |
|
heparin plasma(n=5) |
85-102 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-105% |
86-101% |
89-100% |
|
EDTA plasma(n=5) |
87-101% |
84-92% |
82-101% |
|
heparin plasma(n=5) |
81-99% |
89-99% |
82-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
