FineTest
SKU:ER0036
Rat IL-18(Interleukin-18) ELISA Kit
Rat IL-18(Interleukin-18) ELISA Kit
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Product Name
Rat IL-18(Interleukin-18) ELISA Kit
Alias
IL-18 ELISA Kit, Interleukin 18 ELISA Kit, IL18 ELISA Kit, IGIF ELISA Kit, IL-1g ELISA Kit, IL-1F4 ELISA Kit, Interferon-gamma-inducing factor ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-18 ELISA Kit allows for the in vitro quantitative determination of IL-18 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-18 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-18 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-101 |
96 |
|
EDTA plasma(n=5) |
88-103 |
94 |
|
heparin plasma(n=5) |
89-105 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-98% |
87-105% |
87-103% |
|
EDTA plasma(n=5) |
82-95% |
82-88% |
87-96% |
|
heparin plasma(n=5) |
81-88% |
80-97% |
83-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
